DNA Assembly in 3D Printed Fluidics

DNA Assembly in 3D Printed Fluidics

3D-Printing of Milli and Microfluidics for Synthetic Biology Applications
2014-2015
MIT Media Lab

William Patrick,Steven Keating,Prof. Neri Oxman

DNA assembly is a foundational technology for synthetic biology. Microfluidics present an attractive solution for miniaturizing assembly reagent volumes, enabling multiplexed reactions, and automating protocols by integrating multiple protocol steps. However, microfluidics fabrication and operation can be expensive and requires expertise, limiting access to the technology. With advances in commodity digital fabrication tools, it is now possible to directly print fluidic devices and supporting hardware. 3D printed micro and millifluidics are inexpensive, easy to make and quick to produce. We demonstrated Golden Gate DNA assembly in 3D printed fluidics with reaction volumes as small as 490 nL, channel widths as fine as 220 micrometers, and per unit part cost ranging from $0.61 to $5.71.  Quick turnaround and inexpensive material costs allowed for rapid exploration of device parameters, demonstrating a new manufacturing paradigm for design and fabricating hardware for synthetic biology.

In collaboration with Alec A K Nielsen, Taylor Levy, Che-Wei Wang, Jaime J Rivera, Oc- tavio Mondragón-Palomino, Peter A Carr, Prof. Christopher A Voigt, and David S Kong.

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